Prostate Cancer KD in vitro 2R vial
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Prostate Cancer KD in vitro 2R vial

QGel® Prostate KD Matrix, is well-suited for research applications involving prostate cancer cell lines.  The unique combination of adhesion ligands, degradation kinetics and matrix stiffness properties of the QGel® Prostate KD Matrix have been found to favor many prostate cancer cell lines, including H660.  The 2R vial format is ideal for bench work. 

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FEATURE BENEFITS OF QGEL MICROENVIRONMENT SPECIFIC MATRICES

Fully Synthetic

Each hydrogel-based QGel matrix is developed by modulating various properties of the extra-cellular microenvironment (ECM) including cell adhesion-promoting ligands, matrix degradation kinetics initiated by natural cell secretion and ECM stiffness characteristics. The result is a unique synthetic ECM designed to recapitulate in vivo-like cell behavior and tissue growth. This formulation is free of growth factors or any cell culture media ingredients.

 

Reproducible

Because QGel ECM matrices are developed with fully synthetic bioactive components, there is no variability from batch to batch and there is no contamination risk.  This means you can focus your research efforts to achieve the desired endpoints of your research study.

 

A Complete Discovery Solution:

Incorporating the full line of QGel products in your research means that your assets can move upstream and downstream without the need to discover new platform technologies at each step of the way.

integrated solutions 

High Throughput Screening (HTS) Compatible

Once your research has been validated and ready to move downstream, you will be able to upscale your research seamlessly with our high throughput friendly QGel Assay Kits containing the same QGel® Matrix formulation.

 

In Vitro to In Vivo Research

Each in vitro QGel formulation has a companion formulation developed for in vivo animal studies.  QGel matrices offer highly effective solutions for achieving even the most difficult to grow tumor xenograft development in animals.

 

Easy Handling

Easy to follow protocols are included with each QGel product. Just add QGel Buffer and your cell solution to the QGel lyophilized powder and the hydrogel matrix will form within minutes under normal temperature conditions.  Click here to view a video of encapsulating cells with the QGel® Matrix formulation.

 

High Content Data Analysis

Image Analysis

  • -Number of spheroids
  • -Spheroid size distribution
  • -Spheroid morphology
  • -Immunofluorescence

Biomarker

  • -Protein expression
  • -RNA & DNA expression

Proliferation

  • -Metabolic activity
  • -DNA

 

Storage

QGel has a shelf life of 5 years. Store at -20C.

 

Organ Prostate
Disease Cancer
Platform In Vitro
Use Benchwork, Manual
Optimized for Cell Line H660
Gel Volume 500 µL per 2R vial
Product Type QGel 3D Matrix Products

Included with each purchase of a QGel Product is service customer support for incorporating QGel into your workflows.  Below are the most common protocols needed for this product.  Please do not hesitate to contact us if you require assistance. 

 

PROTOCOLS

96-well plate format

N.1.A Cell Encapsulation and Culture- 96-well format

PDF Download: Cell Encapsulation and Culture protocol - 96-well plate format

Video: 

 

N.1.B Cell Encapsulation and Culture: Media Addition- 96-well format

PDF Download: Cell Encapsulation and Culture protocol - 96-well plate format

Video: 

 

N.1.C Cell Encapsulation and Culture: Media Change- 96-well format

PDF Download: Cell Encapsulation and Culture protocol - 96-well plate format

Video: 

 

N.2.1.A Cell Proliferation AlamarBlue® Assay- 96-well format

PDF Download: Cell Proliferation AlamarBlue® protocol - 96-well plate format

Video: 

 

N.2.1.B Cell Proliferation AlamarBlue® Assay: Gel Wash- 96-well format

PDF Download: Cell Proliferation AlamarBlue® protocol - 96-well plate format

Video: 

 

N.3 Cell Fixation for Staining Assay- 96-well format

PDF Download: Cell Fixation for Staining Assay protocol - 96-well plate format

Video: 

 

Gel Disc format

D.1.A Cell Encapsulation and Culture- Gel Disc format

PDF Download: Cell Encapsulation and Culture protocol - Gel Disc format

Video: 

 

D.1.B Disc Transfer to 48-well plate- Gel Disc format

PDF Download: Cell Encapsulation and Culture protocol - Gel Disc format

Video: 

 

D.1.C Cell Encapsulation and Culture: Media Change- Gel Disc format

PDF Download: Cell Encapsulation and Culture protocol - Gel Disc format

Video: 

 

D.2.1.A Cell Proliferation AlamarBlue® Assay- Gel Disc format

PDF Download: Cell Proliferation AlamarBlue® protocol - Gel Disc format

Video: 

 

D.2.1.B Cell Proliferation AlamarBlue® Assay: Gel Wash- Gel Disc format

PDF Download: Cell Proliferation AlamarBlue® protocol - Gel Disc format

Video: 

Frequently Asked Questions

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