Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit
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Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit

Generic Formulation - NON-Degradable WITHOUT RGD peptides - in vitro Assay Kit

QGel® GENERIC Formulation NSC4EN562R adapts to many cell types.

No- MMP Degradable

No- RGD Peptide, cell adhesion properties

The 3D Matrix formulaiton is Non-MMP Degradable and contains NO RGD peptides for cell adhesion.  This formulation well-suited for research applications involving many cancer cell lines.  The Assay Kit format is ideal for high throughput screening. 

Encapsulation Video 

QUOTE BY REQUEST

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The QGel® Assay Kit has been developed for high-throughput cell-based assays and is compatible with existing standard liquid-handling robots.  Each kit comes with protocols tailored to your research needs. Standard cell suspension is added to the Assay Kit to resuspend the lyophilized powder gel formulation and the combined mixture of cell suspension and QGel Matrix is then further dispensed into the separate assay plates.  Most of our clients work with 384-well assay plates, but we are able to help you establish your own protocols for 96-well or 1536-well assay plates as well.

 

This video demonstrates how cells are encapsulated with QGel Assay Kits.

 

FEATURE BENEFITS OF QGEL MICROENVIRONMENT SPECIFIC MATRICES

 

Fully Synthetic

Each hydrogel-based QGel Matrix is developed by modulating various properties of the extra-cellular microenvironment (ECM) including cell adhesion-promoting ligands, matrix degradation kinetics initiated by natural cell secretion and ECM stiffness characteristics. The result is a unique synthetic ECM designed to recapitulate in vivo-like cell behavior and tissue growth. This formulation is free of growth factors or any cell culture media ingredients.

 

Reproducible

Because QGel ECM Matrices are developed with fully synthetic bioactive components, there is no variability from batch to batch and there is no contamination risk.  This means you can focus your research efforts to achieve the desired endpoints of your research study.

 

A Complete Discovery Solution:

Incorporating the full line of QGel products in your research means that your assets can move upstream and downstream without the need to discover new platform technologies at each step of the way.

integrated_solutions 

In Vitro to In Vivo Research

Each in vitro QGel formulation has a companion formulation developed for in vivo animal studies.  QGel Matrices offer highly effective solutions for achieving even the most difficult to grow tumor xenograft development in animals.

 

Easy Handling

Easy to follow protocols are included with each QGel Assay Kit and our dedicated lab technicians provide the assistance necessary to ensure the assays are developed correctly at your labs.   

QGel Assay Kits work with any standard liquid-handling robot.  Simply add the cell suspension with the lyophilized powder in the QGel Assay Kit, resuspend and dispense into your assay plates. The hydrogel matrix will form within minutes under normal temperature conditions with the cells encapsulated. 

Click here to view a video of encapsulating cells with the QGel Assay Kits.

Click here to view a video on drug screening with QGel Matrices at various timepoints after cell encapsulation.

 

High Content Data Analysis

Image Analysis

  • -Number of spheroids
  • -Spheroid size distribution
  • -Spheroid morphology
  • -Immunofluorescence

Biomarker

  • -Protein expression
  • -RNA & DNA expression

Proliferation

  • -Metabolic activity
  • -DNA

 

Storage

QGel has a shelf life of 5 years. Store at -20C.

Organ -Non organ-specific
Disease Cancer
Platform In Vitro
Use Automated, Robotic
Optimized for Cell Line -Generic / Non-cell specific
Gel Volume 100 µL per mini vial
Product Type QGel 3D Matrix Products

Included with each purchase of a QGel Assay Kit is service customer support for translating QGel Assay Kits into your workflow.  Below are the most common protocols needed for this product.  Please do not hesitate to contact us if you require assistance. 

 

PROTOCOLS

T.1.A Cell Encapsulation and Culture- 384-well format

PDF Download: Cell Encapsulation and Culture protocol - 384-well plate format

Video:

 

T.1.B Cell Encapsulation and Culture: Media Addition- 384-well format

PDF Download: Cell Encapsulation and Culture protocol - 384-well plate format

Video:

 

T.1.C Cell Encapsulation and Culture: Media Change- 384-well format

PDF Download: Cell Encapsulation and Culture protocol - 384-well plate format

Video:

 

T.2.1.A Cell Proliferation AlamarBlue® Assay- 384-well format

PDF Download: Cell Proliferation AlamarBlue® protocol - 384-well plate format

Video:

 

T.2.1.B Cell Proliferation AlamarBlue® Assay: Gel Wash- 384-well format

PDF Download: Cell Proliferation AlamarBlue® protocol - 384-well plate format

Video:

 

T.2.2.A Calcein AM Cell Viability Assay- 384-well format

PDF Download: Calcein AM Cell Viability Assay protocol - 384-well plate format

Video:

 

T.2.2.B Calcein AM Cell Viability Assay: Gel Wash- 384-well format

PDF Download: Calcein AM Cell Viability Assay protocol - 384-well plate format

Video:

Frequently Asked Questions

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What is QGel made of?

QGel Matrices are PEG (polyethylen glycol) based material. This polymer is crosslinked into a 3D network with peptides that contain an amino acid sequence that can be cleaved by cell-secreted proteases such as MMPs. Therefore, like in natural matrices, cells are able to degrade the matrix locally via secretion and activation of proteases (MMPs), move further and proliferate. Furthermore, if needed, QGel provides gels with covalently-bond RGD peptides to stimulate cell-integrin binding and allow adhesion of the cells to their environment.