Three-dimensional organoid constructs serve as increasingly widespread in vitro models for development and disease modeling. Current approaches to recreate morphogenetic processes in vitro rely on poorly controllable and ill-defined matrices, thereby largely overlooking the contribution of biochemical and biophysical extracellular matrix (ECM) factors in promoting multicellular growth and reorganization. Here, we show how defined synthetic matrices can be used to explore the role of the ECM in the development of complex 3D neuroepithelial cysts that recapitulate key steps in early neurogenesis. We demonstrate how key ECM parameters are involved in specifying cytoskeleton-mediated symmetry-breaking events that ultimately lead to neural tube-like patterning along the dorsal-ventral (DV) axis. Such synthetic materials serve as valuable tools for studying the discrete action of extrinsic factors in organogenesis, and allow for the discovery of relationships between cytoskeletal mechanobiology and morphogenesis.
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6831-E6839.
Ranga A, Girgin M, Meinhardt A, Eberle D, Caiazzo M, Tanaka EM, Lutolf MP
The Scientist, February 22, 2018
Nature Medicine. Sept 2017; Vol 23, Nr 9: 1028-1035
Cell. 2018 Jan 11;172(1-2):373-386.
Trends Mol Med. 2017 May;23(5):393-410.
Nature. 2016 Nov 24;539(7630):560-564.
Nat Mater. 2016 Mar;15(3):344-52
Subscribe to our newsletter
Stay up-to-date on the latest advancements in 3D cell culture technology