Background

•Drugs targeted to ET receptors render tumors sensitive to conventional chemotherapy in vivo
•Target expressed in vivo, but scientists unable to get target expression in an in vitro model

 

Study Design

•Brain cancer cell line LN229
•Expression of Endothelin receptors (ETAR / ETBR ) as target physiological features

ECM QGel Design

•Multiple microenvironments & culture conditions were screened for this cancer cell line

Result

•QGel identified a synthetic ECM that resulted in the expression of ET receptors, measured by image analysis of tumor spheroids showing intracellular calcium release upon receptor engagement by Endothelin-1 (ET-1)

 

Data – Results

 

Proliferation measured by metabolic activity over 25 days

 

Several gels yield growth

but…

Only synthetic ECM with specific integrin-binding sites promotes the expression of a target receptor.

 

 

 

 

 

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