QGel Protocols for 2R vials

Included with each purchase of a QGel product is service customer support for translating QGel assays into your workflow.  Below are the most common protocols needed for this product.  Please do not hesitate to contact us if you require assistance.

96-Well Plate Format

 

N.1 Cell Encapsulation and Culture- 96-well format

PDF Download: N.1 Cell Encapsulation and Culture Protocol_QGel-96wp format_v1.6

Video Protocol part. 1/3: N.1.A Cell Encapsulation

Video Protocol part 2/3: N.1.B Media Addition

Video Protocol part 3/3: N.1.C Media Change

N.2.1 Cell Proliferation AlamarBlue Assay- 96-well format

PDF Download: N.2.1 Cell Proliferation AlamarBlue Assay Protocol_QGel-96wp format_v4.5

Video Protocol part 1/2: N.2.1.A Cell Proliferation AlamarBlue Assay

Video Protocol part 2/2: N.2.1.B Gel Wash

N.2.2 Calcein AM Cell Viability Assay- 96-well format

PDF Download: N.2.2 Calcein AM Cell Viability Assay Protocol_QGel-96wp format_v1.1

N.3.1 Cell Fixation for Staining Protocol

PDF Download: N.3.1 Cell Fixation in gel Protocol_QGel-96wp format_v1.2

Video Protocol: N.3 Cell Fixation for Staining

N.3.2 Cell Permeabilization and Staining Protocol

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N.4.1 Cell Extraction for RT-qPCR Protocol

PDF Download: N.4.1 Cell Extraction Protocol for RT-PCR_QGel-96wp format_w Appendix-v2.3

N.4.2 Cell Extraction for Passaging Protocol

PDF Download: N.4.2 Cell Extraction for Passaging Protocol_QGel-96wp format_v1.1

 

Disc Format

 

D.1 Cell Encapsulation and Culture- 96-well format

PDF Download: D.1 Cell Encapsulation and Culture Protocol_QGel-disc format_v1.8

Video Protocol part. 1/3: D.1.A Cell Encapsulation

Video Protocol part 2/3: D.1.B Disc Transfer to 48-well plate

Video Protocol part 3/3: D.1.C Media Change

D.2.1 Cell Proliferation AlamarBlue Assay- 96-well format

PDF Download:D.2.1 Cell Proliferation AlamarBlue Assay Protocol_QGel-disc format_v1.5

Video Protocol part 1/2: D.2.1.A Cell Proliferation AlamarBlue Assay

Video Protocol part 2/2: D.2.1.B Gel Wash

D.2.2 Calcein AM Cell Viability Assay- 96-well format

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D.3.1 Cell Fixation for Staining Protocol

PDF Download: D.3.1 Cell Fixation in gel Protocol_QGel-disc format_v1.3

D.3.2 Cell Permeabilization and Staining Protocol

PDF Download: D.3.2 Cell Staining in gel for nuclei and actin filaments Protocol_QGel-disc format_v1.2

D.4.1 Cell Extraction for RT-qPCR Protocol

PDF Download: D.4.1 Cell Extraction for RT-qPCR Protocol_QGel-disc format_v1.2

D.4.2 Cell Extraction for Passaging Protocol

PDF Download: D.4.2 Cell Extraction for Passaging Protocol_QGel-disc format_v1.2

D.5.1 Frozen blocks for histology Protocol

PDF Download: D.5.1 Frozen blocks for histology Protocol_QGel-disc format_v1.4