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> Myoblasts
MOUSE MYOBLASTS in 3D vs 2D
Myoblasts cultured in 3D (Figure 1) within degradable QGel™ matrix with RGD (REF 1001) were compared with 2D culture (Figure 2) on tissue culture plastic. Thanks to the degradability of QGel™ Matrix and the integrin binding peptide RGD, myoblasts proliferated and fused to form differentiated and elongated structures similar to natural in vivo morphology. In contrast, in 2D, the cells remain flat-shaped and grow in a uniform monolayer.
MATERIALS
- Mouse myoblasts (C2C12 cell line)
- Approx. cell seeding density: 300'000 cells/mL gel
- QGel™ MT 3D Matrix: degr. with RGD (REF 1001)
- Culture medium: DMEM + 10% FBS
METHODS
- Encapsulating cells & casting gel discs
- Staining for confocal microscopy
REFERENCE
Case courtesy of:
Aurélien Lathuilière (Prof. Aebischer lab)
Neurodegenerative Studies Laboratory
Brain Mind Institute, FSV
Swiss Institute of Technology (EPFL)
Lausanne, Switzerland.
unpublished data 2010
Figure 1: Confocal image showing cell growth and organization in early muscle fiber structures when cultured for 4 weeks within degradable QGel™ MT 3D Matrix with RGD (REF 1001). Red: CellTracker CMPTX. Blue: DAPI.
Figure 2: Light microscope image of a culture of myoblasts in 2D after 7 days showing cell growth in monolayer (non-natural morphology).
