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STEM CELLS
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CANCER CELLS
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CELL BIOLOGY
> Fibroblasts
Nucleus Pulposus
REGENERATIVE MEDECINE
Myoblasts
FIBROBLASTS cultured in 3D
- in degradable vs non-degradable matrices -
Human foreskin fibroblasts (hFFs) were encapsulated in degradable (Figure 1) and non-degradable (Figure 2) QGel™ matrices for 3 weeks. Differences in cell morphology and proliferation were studied.
Fibroblast viability was close to 100% after 3 weeks culture in degradable QGel™ matrix with the incorporated integrin-binding RGD peptide (REF 1001).
MATERIALS
- Human foreskin fibroblasts
(hFFs, passage 12)
- Approx. cell seeding density: 120'000 cells/mL gel
- QGel™ MT 3D Matrix: degr. w/ RGD (REF 1001)
- QGel™ MT 3D Matrix: non-degr. w/ RGD(REF1007)
- Culture medium: DMEM + 10% FBS
METHODS
- Encapsulating cells & casting gel discs
- Staining for confocal microscopy
REFERENCE
QGel Case:
C. Gandar, S. Rizzi, N. Vannini
QGel, Lausanne, Switzerland.
Data on file 2010
Figure 1: hFFs spread and proliferate when encapsulated in degradable QGel™ MT 3D Matrix (REF 1001). After 3 weeks culture,
they start forming a 3D cellular network. Confocal image of the fibroblasts stained for F-actin filaments (green) and nuclei (red).
Figure 2: When encapsulated in non-degradable QGel™ MT 3D Matrix (REF 1007) for 3 weeks, hFFs show thin processes coming out but remain round shaped and do not proliferate. Confocal image of the fibroblasts stained for F-actin filaments (green) and nuclei (red).
