CASES

STEM CELLS
Mesenchymal Stem Cells

CANCER CELLS
Breast Cancer
Ovarian Cancer
Colon Cancer
> Prostate Cancer

CELL BIOLOGY
Fibroblasts
Nucleus Pulposus

REGENERATIVE MEDECINE
Myoblasts

PROSTATE CANCER CELLS in 3D

Prostate cancer cells were encapsulated within degradable QGel™ matrix with RGD (REF 1001). After a few days culture, single cells were already observed to grow into multicellular spheroids (Fig. 1).

MATERIALS

- Prostate cancer cells (PC3 mCherry cell line)
- Approx. cell seeding density: 500'000 cells/mL gel
- QGel™ MT 3D Matrix: degr. with RGD (REF 1001)
- Culture medium: DMEM + 10% FBS

METHODS

- Encapsulating cells & casting gel discs

REFERENCE

Case courtesy of:
Colette Bichsel, from Prof. Lutolf lab (LSCB)
Institute of Bioengineering, FSV
Swiss Institute of Technology (EPFL)
Lausanne, Switzerland.
unpublished data 2011

Figure 1: Confocal image of prostate cancer cells growing into spheroids within degradable QGel™ MT 3D Matrix with RGD (REF 1001) after 5 days. Red: mCherry fluorescent proteins. Scale bar = 100um.

Figure 2: Light microscope image of prostate cancer cells spheroids grown in QGel matrix after 7 days.