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CANCER CELLS
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Prostate Cancer
CELL BIOLOGY
Fibroblasts
Nucleus Pulposus
REGENERATIVE MEDECINE
Myoblasts
COLON CANCER CELLS in 3D
Colon cancer cells were cultured in 3D within degradable QGel™ matrix with RGD (REF 1001) for three weeks and grew from single cells to large spheroids. Cell division process throughout the spheroids was assessed using Phospho-HistoneH3 staining (Figure 1, red spots).
MATERIALS
- Colon cancer cells (DLD-1 CRC cell line)
- Approx. cell seeding density: 120'000 cells/mL gel
- QGel™ MT 3D Matrix: degr. with RGD (REF 1001)
- Culture medium: RPMI + 10% FBS
METHODS
- Encapsulating cells & casting gel discs
- Staining for confocal microscopy
REFERENCE
Case courtesy of:
Dr. Stavros Milatos and Simone Ragusa
Prof. Tatiana Petrova Laboratory
Centre Pluridisciplinaire d'Oncologie
University of Lausanne
Switzerland
unpublished data 2011
Figure 1: Confocal image of DLD-1 colon cancer cells grown into spheroids within degradable QGel™ MT 3D Matrix with RGD (REF 1001) after 3 weeks. Green: F-actin filaments. Red: Phospho Histone H3. Blue: nuclei.
Scale bar = 50um.
