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CANCER CELLS
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CELL BIOLOGY
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Nucleus Pulposus
REGENERATIVE MEDECINE
Myoblasts
BREAST CANCER CELLS in 3D vs 2D
Breast cancer cells were cultured in 3D (Figure 1) within degradable QGel™ matrix with RGD (REF 1001) and,
in parallel, cultured in 2D (Figure 2) on tissue culture plastic. In 2D the cells formed a monolayer, while, in 3D
cell grew as spheroids demonstrating a growth behavior more similar to in vivo conditions.
MATERIALS
- Breast cancer cells
- Approx. cell seeding density: 350'000 cells/mL gel
- QGel™ MT 3D Matrix: degr. with RGD (REF 1001)
- Culture medium: DMEM + 10% FBS
METHODS
- Encapsulating cells & casting gel discs
- Staining for confocal microscopy
REFERENCE
Case courtesy of:
Dr. Girieca Lorusso (Prof Rüegg Lab)
Pathology, Department of Medicine
Faculty of Science, University of Fribourg
Switzerland
unpublished data 2010
Figure 1: Breast cancer cells cultured in 3D in QGel™ matrix show cancer cell spheroids formation. Confocal image of breast cancer cells grown in 3D within degradable QGel™ MT 3D Matrix with RGD (REF 1001) after 18 days. Green: F-actin filaments. Red: nuclei. Scale bar = 100um.
Figure 2: Breast cancer cells cultured in 2D show cell growth in monolayer, a non-in vivo-like morphology. Light microscope image of cancer cells grown in 2D on a conventional plastic flask surface after 7 days.
